California Registered Dental Assistant (RDA) Written Practice Exam

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Which of the following steam autoclave settings and times are most effective for sterilization of wrapped instruments?

  1. 134 degrees C for 5 minutes

  2. 121 degrees C (250 degrees F) and 15 psi for 30 minutes

  3. 160 degrees C for 2 hours

  4. 100 degrees C for 60 minutes

The correct answer is: 121 degrees C (250 degrees F) and 15 psi for 30 minutes

Steam autoclaves use a combination of high temperature and pressure to effectively sterilize instruments. Option A and D have either a lower temperature or shorter time, which may not fully sterilize the instruments. Option C has a higher temperature, but a longer time may not be necessary and can potentially damage the instruments. Option B has the optimal balance of temperature and time for effective sterilization.